Template Guided DNA

Description:

Princeton University Invention # 09-2521

 

Researchers in Ecology and Evolutionary Biology and Molecular Biology, Princeton University, have  described a new  process based on the natural biological mechanism of RNA-guided DNA recombination as discovered and reported in their recent publication, Nowacki et al. Nature  2008 January ¹.

 

The process describes a general approach to target or program specific DNA or genome rearrangements in a wide range of cells, based just on knowledge of the desired rearrangement product or DNA sequence. This process was initially demonstrated in a single-cell organism, Oxytricha trifallax, but now further data shows that this method can extend to human cancer cells, which opens up a wide range of potential applicability. Princeton researchers used a human pancreatic cancer cell line and tested a DNA rearrangement in a gene associated with breast cancer to show that the phenomenon may be general for most cancers.  Therefore the method offers a novel mechanism for programming rearrangements in many organisms and cell types. 

 

            Possible applications include gene therapy for specific rearrangements, such as programming deletion of an unwanted stretch of DNA or repairing unrearranged DNA. Possible screens for early cancer detection and prevention could also be developed, based on detecting the presence of certain DNA or RNA templates in precancerous or cancerous cells.

 

Princeton is currently seeking commercial partners for the further development and commercialization of this opportunity. Patent protection is pending.

 

References:

 

¹Nowacki M, Vijayan V, Zhou Y, Schotanus K, Doak TG, Landweber LF, RNA-Mediated Epigenetic     Programming of a Genome-Rearrangement  Pathways , Nature, 2008, January 10;451:153-8.

 

Yao, Meng-Chao, RNA Rules, News & Views, Nature, 2008, January 10;451:131.

 

 

NJN News Science Report January 17th, 2008

http://www.youtube.com/watch?v=5YHVP2xDbS0

 

 

For more information on Princeton University invention # 09-2521 please contact:

 

                                Laurie Tzodikov

                                Office of Technology Licensing and Intellectual Property

                                Princeton University

                                4 New South Building

                                Princeton, NJ 08544-0036

                                (609) 258-7256

                                (609) 258-1159 fax

                                tzodikov@princeton.edu

Patent Information:
For Information, Contact:
Laurie Tzodikov
Licensing Associates
Princeton University
tzodikov@princeton.edu
Inventors:
Laura Landweber
Mariusz Nowacki
Wenwen Fang
Yibin Kang
Keywords: